Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein.
Identifieur interne : 000571 ( Main/Exploration ); précédent : 000570; suivant : 000572Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein.
Auteurs : Jehad Shaikhali [Suède] ; Céline Davoine [Suède] ; Kristoffer Br Nnström [Suède] ; Nicolas Rouhier [France] ; Joakim Bygdell [Suède] ; Stefan Björklund [Suède] ; Gunnar Wingsle [Suède]Source :
- The Biochemical journal [ 1470-8728 ] ; 2015.
Descripteurs français
- KwdFr :
- Arabidopsis (métabolisme), Complexe médiateur (génétique), Complexe médiateur (métabolisme), Facteurs de transcription (MeSH), Glutarédoxines (métabolisme), Glutathion (métabolisme), Mutagenèse dirigée (MeSH), Mutation (MeSH), Oxydoréduction (MeSH), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Protéines recombinantes (métabolisme), Régions promotrices (génétique) (MeSH), Sous-unités de protéines (génétique), Sous-unités de protéines (métabolisme), Techniques de double hybride (MeSH), Thiorédoxines (métabolisme), Éléments de réponse (MeSH).
- MESH :
- génétique : Complexe médiateur, Protéines d'Arabidopsis, Protéines de liaison à l'ADN, Sous-unités de protéines.
- métabolisme : Arabidopsis, Complexe médiateur, Glutarédoxines, Glutathion, Protéines d'Arabidopsis, Protéines de liaison à l'ADN, Protéines recombinantes, Sous-unités de protéines, Thiorédoxines.
- Facteurs de transcription, Mutagenèse dirigée, Mutation, Oxydoréduction, Régions promotrices (génétique), Techniques de double hybride, Éléments de réponse.
English descriptors
- KwdEn :
- Arabidopsis (metabolism), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Glutaredoxins (metabolism), Glutathione (metabolism), Mediator Complex (genetics), Mediator Complex (metabolism), Mutagenesis, Site-Directed (MeSH), Mutation (MeSH), Oxidation-Reduction (MeSH), Promoter Regions, Genetic (MeSH), Protein Subunits (genetics), Protein Subunits (metabolism), Recombinant Proteins (metabolism), Response Elements (MeSH), Thioredoxins (metabolism), Transcription Factors (MeSH), Two-Hybrid System Techniques (MeSH).
- MESH :
- chemical , genetics : Arabidopsis Proteins, DNA-Binding Proteins, Mediator Complex, Protein Subunits.
- metabolism : Arabidopsis, Arabidopsis Proteins, DNA-Binding Proteins, Glutaredoxins, Glutathione, Mediator Complex, Protein Subunits, Recombinant Proteins, Thioredoxins.
- Mutagenesis, Site-Directed, Mutation, Oxidation-Reduction, Promoter Regions, Genetic, Response Elements, Transcription Factors, Two-Hybrid System Techniques.
Abstract
The eukaryotic mediator integrates regulatory signals from promoter-bound transcription factors (TFs) and transmits them to RNA polymerase II (Pol II) machinery. Although redox signalling is important in adjusting plant metabolism and development, nothing is known about a possible redox regulation of mediator. In the present study, using pull-down and yeast two-hybrid assays, we demonstrate the association of mediator (MED) subunits MED10a, MED28 and MED32 with the GLABROUS1 (GL1) enhancer-binding protein-like (GeBPL), a plant-specific TF that binds a promoter containing cryptochrome 1 response element 2 (CryR2) element. All the corresponding recombinant proteins form various types of covalent oligomers linked by intermolecular disulfide bonds that are reduced in vitro by the thioredoxin (TRX) and/or glutathione/glutaredoxin (GRX) systems. The presence of recombinant MED10a, MED28 and MED32 subunits or changes of its redox state affect the DNA-binding capacity of GeBPL suggesting that redox-driven conformational changes might modulate its activity. Overall, these results advance our understanding of how redox signalling affects transcription and identify mediator as a novel actor in redox signalling pathways, relaying or integrating redox changes in combination with specific TFs as GeBPL.
DOI: 10.1042/BJ20150132
PubMed: 25877331
Affiliations:
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(genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>DNA-Binding Proteins (genetics)</term><term>DNA-Binding Proteins (metabolism)</term><term>Glutaredoxins (metabolism)</term><term>Glutathione (metabolism)</term><term>Mediator Complex (genetics)</term><term>Mediator Complex (metabolism)</term><term>Mutagenesis, Site-Directed (MeSH)</term><term>Mutation (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Protein Subunits (genetics)</term><term>Protein Subunits (metabolism)</term><term>Recombinant Proteins (metabolism)</term><term>Response Elements (MeSH)</term><term>Thioredoxins (metabolism)</term><term>Transcription Factors (MeSH)</term><term>Two-Hybrid System Techniques (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (métabolisme)</term><term>Complexe médiateur (génétique)</term><term>Complexe médiateur (métabolisme)</term><term>Facteurs de transcription (MeSH)</term><term>Glutarédoxines (métabolisme)</term><term>Glutathion (métabolisme)</term><term>Mutagenèse dirigée (MeSH)</term><term>Mutation (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Protéines d'Arabidopsis (génétique)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Protéines recombinantes (métabolisme)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Sous-unités de protéines (génétique)</term><term>Sous-unités de protéines (métabolisme)</term><term>Techniques de double hybride (MeSH)</term><term>Thiorédoxines (métabolisme)</term><term>Éléments de réponse (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term><term>DNA-Binding Proteins</term><term>Mediator Complex</term><term>Protein Subunits</term></keywords><keywords scheme="MESH" qualifier="génétique" 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Regions, Genetic</term><term>Response Elements</term><term>Transcription Factors</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs de transcription</term><term>Mutagenèse dirigée</term><term>Mutation</term><term>Oxydoréduction</term><term>Régions promotrices (génétique)</term><term>Techniques de double hybride</term><term>Éléments de réponse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The eukaryotic mediator integrates regulatory signals from promoter-bound transcription factors (TFs) and transmits them to RNA polymerase II (Pol II) machinery. Although redox signalling is important in adjusting plant metabolism and development, nothing is known about a possible redox regulation of mediator. In the present study, using pull-down and yeast two-hybrid assays, we demonstrate the association of mediator (MED) subunits MED10a, MED28 and MED32 with the GLABROUS1 (GL1) enhancer-binding protein-like (GeBPL), a plant-specific TF that binds a promoter containing cryptochrome 1 response element 2 (CryR2) element. All the corresponding recombinant proteins form various types of covalent oligomers linked by intermolecular disulfide bonds that are reduced in vitro by the thioredoxin (TRX) and/or glutathione/glutaredoxin (GRX) systems. The presence of recombinant MED10a, MED28 and MED32 subunits or changes of its redox state affect the DNA-binding capacity of GeBPL suggesting that redox-driven conformational changes might modulate its activity. Overall, these results advance our understanding of how redox signalling affects transcription and identify mediator as a novel actor in redox signalling pathways, relaying or integrating redox changes in combination with specific TFs as GeBPL. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25877331</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1470-8728</ISSN><JournalIssue CitedMedium="Internet"><Volume>468</Volume><Issue>3</Issue><PubDate><Year>2015</Year><Month>Jun</Month><Day>15</Day></PubDate></JournalIssue><Title>The Biochemical journal</Title><ISOAbbreviation>Biochem J</ISOAbbreviation></Journal><ArticleTitle>Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein.</ArticleTitle><Pagination><MedlinePgn>385-400</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1042/BJ20150132</ELocationID><Abstract><AbstractText>The eukaryotic mediator integrates regulatory signals from promoter-bound transcription factors (TFs) and transmits them to RNA polymerase II (Pol II) machinery. Although redox signalling is important in adjusting plant metabolism and development, nothing is known about a possible redox regulation of mediator. In the present study, using pull-down and yeast two-hybrid assays, we demonstrate the association of mediator (MED) subunits MED10a, MED28 and MED32 with the GLABROUS1 (GL1) enhancer-binding protein-like (GeBPL), a plant-specific TF that binds a promoter containing cryptochrome 1 response element 2 (CryR2) element. All the corresponding recombinant proteins form various types of covalent oligomers linked by intermolecular disulfide bonds that are reduced in vitro by the thioredoxin (TRX) and/or glutathione/glutaredoxin (GRX) systems. The presence of recombinant MED10a, MED28 and MED32 subunits or changes of its redox state affect the DNA-binding capacity of GeBPL suggesting that redox-driven conformational changes might modulate its activity. Overall, these results advance our understanding of how redox signalling affects transcription and identify mediator as a novel actor in redox signalling pathways, relaying or integrating redox changes in combination with specific TFs as GeBPL. </AbstractText><CopyrightInformation>© The Authors Journal compilation © 2015 Biochemical Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shaikhali</LastName><ForeName>Jehad</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences SLU, 901 83 Umeå, Sweden jehadgermany@gmail.com Gunnar.Wingsle@slu.se.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davoine</LastName><ForeName>Céline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences SLU, 901 83 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brännström</LastName><ForeName>Kristoffer</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Medical Biochemistry and Biophysics, Umeå Plant Science Center, Umeå University, SE-901 87 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rouhier</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500 Vandoeuvre-lès-Nancy, France INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280 Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bygdell</LastName><ForeName>Joakim</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences SLU, 901 83 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Björklund</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Medical Biochemistry and Biophysics, Umeå Plant Science Center, Umeå University, SE-901 87 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wingsle</LastName><ForeName>Gunnar</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences SLU, 901 83 Umeå, Sweden jehadgermany@gmail.com Gunnar.Wingsle@slu.se.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biochem J</MedlineTA><NlmUniqueID>2984726R</NlmUniqueID><ISSNLinking>0264-6021</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056892">Mediator Complex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021122">Protein Subunits</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000629467">STKR1 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>144417-31-2</RegistryNumber><NameOfSubstance UI="C071556">GL1 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>52500-60-4</RegistryNumber><NameOfSubstance UI="D013879">Thioredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056892" MajorTopicYN="N">Mediator Complex</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021122" MajorTopicYN="N">Protein Subunits</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020218" MajorTopicYN="N">Response Elements</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013879" MajorTopicYN="N">Thioredoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020798" MajorTopicYN="N">Two-Hybrid System Techniques</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis thaliana</Keyword><Keyword MajorTopicYN="N">CryR2</Keyword><Keyword MajorTopicYN="N">GLABROUS1 enhancer-binding protein (GeBP)</Keyword><Keyword MajorTopicYN="N">deoxyribonucleic acid (DNA)-binding</Keyword><Keyword MajorTopicYN="N">mediator</Keyword><Keyword MajorTopicYN="N">redox</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>01</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25877331</ArticleId><ArticleId IdType="pii">BJ20150132</ArticleId><ArticleId IdType="doi">10.1042/BJ20150132</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>Suède</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Champenoux</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="Suède"><noRegion><name sortKey="Shaikhali, Jehad" sort="Shaikhali, Jehad" uniqKey="Shaikhali J" first="Jehad" last="Shaikhali">Jehad Shaikhali</name></noRegion><name sortKey="Bjorklund, Stefan" sort="Bjorklund, Stefan" uniqKey="Bjorklund S" first="Stefan" last="Björklund">Stefan Björklund</name><name sortKey="Br Nnstrom, Kristoffer" sort="Br Nnstrom, Kristoffer" uniqKey="Br Nnstrom K" first="Kristoffer" last="Br Nnström">Kristoffer Br Nnström</name><name sortKey="Bygdell, Joakim" sort="Bygdell, Joakim" uniqKey="Bygdell J" first="Joakim" last="Bygdell">Joakim Bygdell</name><name sortKey="Davoine, Celine" sort="Davoine, Celine" uniqKey="Davoine C" first="Céline" last="Davoine">Céline Davoine</name><name sortKey="Wingsle, Gunnar" sort="Wingsle, Gunnar" uniqKey="Wingsle G" first="Gunnar" last="Wingsle">Gunnar Wingsle</name></country><country name="France"><region name="Grand Est"><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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